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Segmentos:

FRASER Listeria caldo de enriquecimento selectivo (base)

para microbiologia GranuCult®

Descrição
Número de catálogo110398
Replaces1.10398
Panorama geralGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Informações sobre o produto
Código HS3821 00 00
Quality LevelMQ200

Informações fisico-químicas
Valor do pH7.0 – 7.4 (57.4 g/l, H₂O, 25 °C)
Solubilidade57.4 g/l

Informação de segurança de acordo com o GHS
Classe de armazenagem10 – 13 Outros liquídos e sólidos
WGKWGK 3 forte contaminante da água
Descarte3
Os reagentes organicos líquidos relativamente não reactivos são compilados na Categoria A. Se contiverem halógeneo, devem ser colocados na Categoria B. Para resíduos sólidos: Categoria C.

Informações sobre armazenagem e transporte
ArmazenagemArmazenar entre +15°C a +25°C.

Especificações
Appearance (clearness)clear to almost clear
Appearance (color)yellowish-brown
pH-value (25 °C)7.0 – 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109))≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))≥ 1000
Blacking (Listeria monocytogenes ATCC 13932 (WDCM 00021))+
Blacking (Listeria monocytogenes ATCC 35152 (WDCM 00109))+
Blacking (Escherichia coli ATCC 25922 (WDCM 00013))
Blacking (Escherichia coli ATCC 8739 (WDCM 00012))
Blacking (Enterococcus faecalis ATCC 19433 (WDCM 00009))
Blacking (Enterococcus faecalis ATCC 29212 (WDCM 00087))
Growth on ALOA® medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))> 10 colonies
Growth on ALOA® medium(Listeria monocytogenes ATCC 35152 (WDCM 00109))> 10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021))blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109))blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))< 100 colonien
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))< 100 colonies
Incubation:
Half-Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Fraser Broth: 48 ± 2 hours at 37 ± 1°C
Confirmation on ALOA® medium: 44 ± 4 hours at 37 ±1°C
Confirmation on Tryptic soy agar: 24 ± 3 hours at 37 ± 1°C